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. 2016 Apr 28;11(4):e0152793. doi: 10.1371/journal.pone.0152793

Fig 6. Effect of elastase injection on Fbln5 Cre+cKO mice.

Fig 6

A. Purified porcine elastase was injected into the posterior vaginal wall in Fbln5f/f/SMA++/Cre- (Cre-, open bar, n = 5) and Fbln5f/f/SMA++/Cre+ or Fbln5f/-/SMA++/Cre+ (Cre+, solid bar, n = 9). MOPQ measurements were recorded 48 h after 5, 10, and 15 U of elastase. Since effects of elastase did not differ among Fbln5f/f/SMA++/Cre+ (n = 4) compared with Fbln5f/-/SMA++/Cre+ (n = 5), data were combined as mean ± SEM. Magnitude of bulge shown. *p < 0.05 compared with Cre-. B. Immunoblotting of Fbln5 in urea extracts from elastase-injected vaginal muscularis of Cre- and Cre+cKO mice. F5-/-, negative control; Ctl, nonpregnant wild type; PP, 48 h postpartum wild type control indicating decrease in Fbln5. Coomassie gel indicated even loading (not shown). C. Gelatin zymography of Ctl and cKO vaginal extracts from elastase-treated mice. D. Hart’s stain of posterior vaginal wall of elastase-injected Ctl (a) or cKO (b) mice.