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. 2016 Apr 28;12(4):e1006010. doi: 10.1371/journal.pgen.1006010

Fig 4. Genetic interaction analyses reveals a synthetic lethal interaction between madd-3 and unc-54.

Fig 4

A. Double mutant analyses reveals that madd-3(tr186) enhances the muscle arm defects of all mutants shown. Counts for muscle VL11 are shown (n = 30). B. The viability of unc-54(e190); madd-3(tr186) double mutants is dependent on a rescuing array that expresses either MADD-3 or UNC-54. Shown for each genotype are the fraction of F1 progeny that carry the indicated extra-chromosomal (Ex) array from parents that also carried the same array. All double homozygotes are dependent on the array to become viable fertile adults (see text), while double heterozygotes do not. The array carrying the endogenous promoter expresses YFP::MADD-3A within the context of a 29 kb fosmid clone (construct pPRSAD539- see methods); the myo-3 promoter was used to express MADD-3A specifically in muscles (construct pPRSAD499); UNC-54 was expressed in the muscles from construct pPD5.41 (a gift from Andrew Fire). C. madd-3(tr186) is synthetic lethal with a temperature sensitive allele of unc-54(e1157). Strains were grown at the indicated temperature (Celsius) for at least 3 days before individual L4s were cloned on to separate plates (n = 8). Progeny of the cloned animals were counted when they reached L4. For all graphs shown, an asterisk indicates a significant difference (p<0.05) with respect to the data point indicated with a closed circle of the same color as the asterisks.