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. 2016 Apr 28;12(4):e1006010. doi: 10.1371/journal.pgen.1006010

Fig 5. Mutations in the p38 MAP kinase pathway suppress the synthetic lethality of the unc-54; madd-3 double mutant.

Fig 5

A. The 41 suppressors that we isolated allow the unc-54(e190); madd-3(tr186) double mutants to survive without a rescuing extra-chromosomal array. The average number of progeny thrown by a single adult of the indicated genotype is shown (n = 10). The color of the name of the allele is matched to the color of the MAP kinase components in B-E. B-D. Schematics of the indicated MAP kinase pathway components and the position of the mutations that we identified. The green box indicates the kinase domain. E. A schematic of the MAP kinase pathway. F. Deletion alleles of the p38 MAP kinase pathway suppress the synthetic lethality of the unc-54; madd-3 double mutant. Shown are the fraction of progeny from individual unc-54(e190); madd-3(tr186) double mutants that harbour an extra-chromosomal array that expresses MADD-3A specifically in muscles (from the construct pPRSAD499). The genetic background is indicated. Note that all triple mutant strains survive and propagate without the presence of the extra-chromosomal array. An asterisk indicates a significant difference (p<0.05) compared to the data point indicated with a closed circle of the same color as the asterisks.