Skip to main content
. 2016 Apr 28;12(4):e1006010. doi: 10.1371/journal.pgen.1006010

Fig 7. Mutations in p38 MAP kinase components and the mucolipin ortholog CUP-5 suppress the decrease in abundance of EVA-1 in madd-3 mutants.

Fig 7

A-E. Muscle cells from animals of the indicated genotype express EVA-1:: MYC::3XFLAG::CFP in muscles from the trIs89 chromosomally integrated transgene. Each picture was taken with the same exposure time. F-G. Muscle cells from animals of the indicated genotype express UNC-40::YFP in muscles from the trIs41 transgene. H-I. Muscle cells from animals of the indicated genotype express PAT-2::CFP in muscles from the trIs72 integrated transgene. The scale bars in A-I represent 25μm. J. A western blot of whole worm lysate from a mixed stage population of the indicated genotype probed with an antibody against GFP (which recognizes CFP) (top) and an antibody against tubulin as a loading control (bottom). EVA-1::CFP is expressed from the integrated transgene trIs89. K. Quantification of fluorescent signal for the indicated transgene and genetic background. The transgenic protein whose abundance is being measured is indicated below the genotype at the bottom of the panel. An asterisk indicates a significant difference (p<0.01) compared to the data point indicated with a closed circle of the same color as the asterisks.