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. 2016 Apr 28;12(4):e1006010. doi: 10.1371/journal.pgen.1006010

Fig 8. Mutations in p38 MAP kinase components and the mucolipin ortholog CUP-5 suppress the decrease in abundance of RAB-7 in madd-3 mutants.

Fig 8

A-I. The muscle cells of animals of the indicated genotype expressing the indicated vesicle marker. A-B. mCherry::RAB-5 is specifically expressed in muscles from an extrachromsomal array harboring the pPRSAD950 plasmid. C-F. mCherry::RAB-7 is specifically expressed in muscles from the huIs89 integrated transgene. G-I. mCherry::RAB-11 is specifically expressed in muscles from the huIs97 integrated transgene. All RAB reagents were kind gifts from Rik Korswagen. The scale bars in A-H represent 50 μm. J. Western blot analyses of whole worm lysate from a mixed stage population of the indicated genotype probed with anti-RFP antibodies, which recognize mCherry (top), and anti-tubulin antibodies (bottom) as a loading control. K. Quantification of the RAB-7 vesicle phenotype in the indicated genetic background. L. Quantification of the RAB-11 vesicle phenotype in the indicated genetic background. MADD-3A is specifically in muscles from the myo-3 promoter from an extra-chromosomal array (from the pPRSAD499 plasmid). For K-L, the presence of a wild type or tr186 allele of MADD-3A is indicated with a respective (+) or (-) below the bars of the graph. An asterisk indicates a significant difference (p<0.05) compared to the data point indicated with a closed circle of the same color as the asterisks.