Figure 5.

C-26 tumors induce severe muscle wasting associated with a selective loss of MyHC protein. (A) Saline used as control (100 μl) or C-26 adenocarcinoma cells (1 × 10⁶ cells per 0.1 ml) were injected subcutaneously into the right flank of CD2F1 male mice. Body weights of saline- or C-26–injected mice (n = 4 per group) were recorded every 48 hours from day 8 to day 22 postinjection. (B and C) Mice (n = 6) were injected in the right flank with either saline (control) or C-26 cells. At the indicated days after injection, tibialis anterior muscles from the right hind limb were collected and divided into two sections. From one section, tissue homogenates were prepared, and Western blot analyses probing for myofibrillar proteins were performed (B). From the remaining section, total RNA was prepared, and Northern blot analyses were undertaken (C). (D) Extracts of soleus muscles from mice described in B and C were prepared and used for Western blot analysis for expression of MyHC type I and α-sarcomeric actin.