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. 2016 Apr 28;11(4):e0154373. doi: 10.1371/journal.pone.0154373

Fig 5. CB0313.1 on TJ proteins expression, serum LPS and epididymal adipose inflammation.

Fig 5

(a) Western blot analysis of TJ proteins, claudin-1, occludin and GAPDH as housekeeping protein (n = 3), (b) TJ proteins (claudin-1 and occludin) mRNA expression in mouse colon were determined by RT-qPCR (n = 5). (c) Concentration of LPS in serum were measured by ELISA (n = 5). (d) Epididymal adipose inflammation were determined by the transcriptional levels of pro-inflammatory gene (TNF-α, IL-1β, IL-6 and MCP-1, n = 4). Significant difference between ND versus HFD are indicated as #P<0.05, ##P<0.01, ###P<0.001, while significant difference between HFD and HFD-CB are indicated as *P<0.05, **P<0.01.