Table 2. Modified Kleiner scoring of tissue sections isolated from PF and EtOH PTEN^f/f and PTEN^LKO mice.

		PTENf/f			PTENLKO			Two-Way ANOVA
Parameter╪	PF		EtOH	PF		EtOH	Genotype	EtOH	Interaction
Steatosis (0–3)	0.04±0.12a		0.43±0.13b	2.93±0.13a,c		2.07±0.16b,c	<0.0001	0.0965	0.0206
Ballooning/Degeneration (0–2)	0.0±0.0		0.0±0.0	1.14±0.23c		0.43±0.28c	0.0002	0.0618	0.0618
Lobular Inflammation (0–2)	0.0±0.0		0.0±0.0	1.00±0.0c		1.00±0.0c	<0.0001	1	1
Microsteatosis (0–3)	0.0±0.0		0.0±0.0	1.00±0.0c		1.00±0.0c	0.0001	1	1
Score Modified Kleiner (0–10)	0.04±0.12a		0.43±0.13b	6.07±0.25a,c		4.50±0.30b,c	<0.0001	0.0099	0.0002
Biliary Hyperplasia	0.0±0.0		0.0±0.0	2.00±0.29c		2.14±0.23c	<0.0001	0.7086	0.7068

Overall hepatic steatosis, hepatocyte ballooning, lobular inflammation, microsteatosis, biliary hyperplasia were quantified according to Brunt and Kleiner with minor modifications [30]. Data are means± SEM as analyzed by two-way ANOVA with a Bonferroni post hoc analysis (PTEN^f/f group compared to PTEN^LKO group). To determine specific effects of EtOH within each group, an unpaired student’s t-test was performed. Means without a common superscript letter are significantly different (N = 7 pairs of PTEN^f/f PF/EtOH and 7 pairs of PTEN^LKO PF/EtOH mice/group (p<0.05)). Values in bold indicate significance by two-way ANOVA.

^╪Letter superscripts (a,b,c) denote significant difference of P<0.05 by unpaired t-test, values in bold significant difference by Two-Way ANOVA.