Figure 4.

CD34⁺ cell transplantation accelerates neuronal regeneration after stroke. (A and B) On day 14, animals receiving CD34⁺ cells after stroke displayed migration of NPCs toward the ischemic area by PSA-NCAM immunostaining. (C–E) Analysis of serial sections displayed expression of neuronal stem cell markers, Musashi-1 (C) and DCX (D). Note that expression of DCX was limited to the area proximal to the SVZ. PSA-NCAM⁺ NPCs also expressed NeuN (E). Small NeuN⁺ nuclei were observed in PSA-NCAM⁺ NPCs, whereas more intensely staining and larger nuclei represent mature neurons. (F) On day 14 on the contralateral side, PSA-NCAM⁺ NPCs were limited to the SVZ; that is, no migration of NPCs was observed. (G) Migration of NPCs with small NeuN⁺ nuclei toward cortex was observed in poststroke mice treated with PBS on day 14 after cell transplantation. (H) The average number of NPCs in the white matter at the lower left of the left cortex per HPF from 5 animals under each condition. Three sections were evaluated in each animal, and n = 5 per group. Arrowheads delineate individual NPCs or demarcate areas rich in NPCs. Scale bars: 1 mm (A), 0.2 mm (B and F), and 0.4 mm (G). *P < 0.05 versus PBS.