Fig 3. Serial enrichment for invasive recombinants by gentamicin protection.

(A) Boxplots of intracellular invasion frequencies from Pools 0–4 (2 experiments in triplicate). (B) Boxplots of adhesion frequencies (2 experiments in triplicate). The values show the combined ability of clones in Pool n to invade or adhere to airway epithelial cells, while the recovered colonies comprise Pool n+1. Nal^R and Nov^R pools are summarized by the left and right boxplots, with individual data points shown as circles and squares, respectively.