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. 2016 Apr 28;11(4):e0154384. doi: 10.1371/journal.pone.0154384

Table 1. Candidate reference genes, annotated functions, and primers used to amplify and their PCR parameters.

Gene A. thaliana Gene Description A. Thaliana Ortholog Primer sequences (5’ - 3’) forward/reverse Tm (°C) Amplicon length (bp) Identity (%)
CeCLATHRIN1 clathrin adaptor complexes medium subunit family protein NP_199475.1 CTTCACCACTACTCCCAATG/CCTGATGTCTTCAAAAGGGA 54 415 90
CePP2A serine/threonine-protein phosphatase PP2A-1 catalytic subunit NP_176192.1 GTTATCAAGCGTGTCCAAAG/GAATGTGCAACCTGTGAAAT 54 409 82
CeRPB2 DNA-directed RNA polymerase II subunit RPB2 NP_193902.1 TCTTTTGCAGGTAAGACCTC/ACGAAGATTATGTGGACGAG 52 1248 80
CeGAPC2 glyceraldehyde3-phosphate dehydrogenase GAPC2 NP_001077530.1 GGCCATTCCAGTCAATTTTC/GTCATCCATGAAAGGTTTGG 54 210 94
CeTIP41 TIP41-like protein tonoplast intrinsic protein4(Zea mays) NP_195153.2 AGACAGCTCTCTTGAACTTG/TGAAATGCAAACCAGAATGG 55 900 84
CeMAPK mitogen-activated protein kinase 1 NP_172492.1 GTGATTCTCTTACAGGGGTC/TCATACGGTATCGTCTGTTC 54 803 77
CeSAMDC S-adenosylmethionine decarboxylase NP_001154585.1 AAACCTTAGAAGCCCACAAT/TGTGTTAAGTACACTCGTGG 54 735 74
CeEIF4 eukaryotic initiation factor 4A-3 NP_177417.1 AACCTTCCGAGCTGTATTTT/TCCAGTCCTCCTTATCAACA 52 849 84
CeEF1 elongation factor 1-alpha 2 NP_563800.1 CAGCGAATTTTGAGAAGTGG/GGAACTCATACGAAGTCAGA 55 745 78
CeACT7 actin 7 NP_196543.1 GGAGGTGCTACAACCTTTAT/TGATGGAGTTACACACACTG 53 531 99
CeTUB tubulin alpha-6 chain NP_193232.1 GTTGGCCTCTCAATATCCAA/AATCCCTCTTCAGACCTCAT 55 731 80
CeUBQ ubiquitin 11 NP_567286.1 GACGGGAAAGACCATAACTT/TGTGAATATAAGCCAGCGAT 52 400 81
CeCYP Cyclophilin peptidyl-prolyl cis-trans isomerase CYP19-2 NP_179709.1 AAAATCCCAGGGTGTTCTTG/ATTTCACCGCTGTCTACAAT 55 511 81