Figure 3.

DSCG administration prior to MPO immunization, as a preventative, protected from the development of GN. Renal injury assessed by albuminuria (A), glomerular segmental necrosis (B and C) and glomerular leukocyte accumulation (D) was attenuated in mice that received DSCG (n=8), compared with saline treated controls (n=7). Kidney MCs were identifiable in controls and significantly reduced by DSCG to numbers similar to those observed in naïve C57BL/6 mice (dotted line; n=5) (E). Kidney MCs were identified and quantified following methyl Carnoy’s fixation and Berberine sulfate staining, white arrow (F). Error bars represent mean±SEM with statistical analysis by Mann–Whitney t-test. **P<0.01, *P<0.05.