Figure 1.

Transcript expression of creatine transporters MCT12 and CRT1 along the mouse nephron. Indicated segments were microdissected, total RNA isolated and reverse transcribed. Expression of indicated transcripts was quantified by Taqman-based RT-PCR and normalized to GAPDH transcript levels. Upper panel: segment-specific transcripts and purity of preparation. Lower panel: expression of MCT12 and CRT1 transcripts. n=6 mice; data are means±SD. AQP2, aquaporin-2; CCD, cortical collecting duct; CNT, connecting tubule; DCT, distal convoluted tubule; NaPi-2a, sodium/phosphate cotransporter isoform 2a; NCC, Na- and Cl-cotransporter; PCT, proximal convoluted tubule; PST, proximal straight tubule; TALH, thick ascending limb of loop of Henle; UMOD, uromodulin.