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. 2015 Sep 16;27(5):1426–1436. doi: 10.1681/ASN.2015040411

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Figure 2. — Subcellular localization of MCT12 in mouse kidney. Confocal image of a kidney cryosection (A) and of an isolated proximal convoluted tubule (B) stained with anti-MCT12 antibody. Asterisks indicate proximal tubules. Nuclei were counterstained with DAPI (blue, right panel). White scale bar corresponds to 50 µm. (C) Immunoblot of TM or BBM preparation stained with an anti-MCT12 antibody and a basolateral (NHE1) or apical (NaPi-2a) membrane protein. BBM, brush border membrane; G, Glomerulus; NaPi-2a, sodium/phosphate cotransporter isoform 2a; NHE1, sodium/hydrogen exchanger isoform 1; TM, total kidney membrane preparation.