Figure 5.

Human MCT12 does not transport guanidinoacetate. Transport assay in Xenopus oocytes. (A) Immunoblot of cell lysates or surface biotin fractions of Xenopus oocytes 2 days after injection with (untagged) human MCT12 cRNA or H₂O only. (B) Time course of MCT12-mediated creatine uptake at 100 µM creatine concentration 2 days after injection. (C) Competition of MCT12-mediated creatine uptake at 1 µM creatine (left panel) or 10 and 100 µM creatine (right panel), with or without 100 µM guanidinoacetate (GA) 2 days after injection. Data are means±SD.