Fig. 6.
DARPP-32 expression decreased in the striatum of Hdh+/Q175;p75−/− mice compared to Hdh+/+;p75+/+ mice. (A) WCLs from a half of the striata of the stated genotypes at 5 months of age were probed for the listed antibodies. (B). Quantification of (A). (C–F) Immunohistochemistry was performed on the striata of 7-month-old mice of the stated genotype for DARPP-32 as described in the methods at 10×. (G–J) Images at 64×. Decreased levels of DARPP-32 were observed with WB and IHC in the striatum of Hdh+/Q175;p75−/− mice compared to Hdh+/+;p75+/+, Hdh+/Q175×;p75+/+, and Hdh+/+;p75−/− littermates.