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. Author manuscript; available in PMC: 2016 Sep 21.
Published in final edited form as: Nat Plants. 2016 Mar 21;2:16025. doi: 10.1038/nplants.2016.25

Figure 5. DR5::GUS intensity and distribution indicate that vas2 mutant accumulates more free IAA, which is distributed to the epidermis of hypocotyl under shade regardless of NPA treatment.

Figure 5

DR5::GUS was introduced into sav3-1 mutant, vas2-2 mutant and vas2-2 sav3-1 double mutants by genetic cross. a–h, vas2 mutants exhibit stronger DR5::GUS activities under both Wc and shade. Plants harbouring the DR5::GUS transgene were grown on 1/2 MS plates (−NPA) under Wc for 5 days, and then kept in Wc (a–d) or transferred to shade (e–h) for 4 days. i–l, NPA treatment inhibits DR5::GUS accumulation in the hypocotyl of WT plants, but not of vas2 mutants. Plants harbouring the DR5::GUS transgene were grown on 1/2 MS plates supplemented with 4 μM NPA (+NPA) under Wc for 5 days, and then transferred to shade (i–l) for 4 days.