FIGURE 5:

The Rga6 polybasic C-terminal region is necessary for its membrane localization. (A) Schematic representation of several Rga6 protein truncations. (B) Expression levels of wild-type Rga6-GFP and the truncations shown in A tagged with GFP at the C-terminal region, determined by Western blot. Actin levels were used as loading control (bottom). (C) Localization of the Rga6-GFP and the GFP-tagged protein truncated versions. The exposition time of the Rga6-GFP picture is two times longer than the pictures corresponding to Rga6-GFP truncations to allow a better comparison of the localization. Bar, 5 μm. (D) Box plots representing the cellular dimensions (length, left; and width, right) of strains carrying the indicated truncated Rga6 versions compared with the wild-type and rga6Δ cells. The black dot in each box represents the median, the upper and lower limits of the box represent the 25th and 75th percentiles, respectively, and the whiskers represent the minimum and maximum values. ***p < 0.0001; n.s., not significant difference relative to wild type; Student’s t test.