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Rga6 effect on cell morphology depends on the Cdc42 GEF Scd1. (A) DIC images from wild-type, rga6Δ, rga4Δ, scd1Δ, scd1Δ rga6Δ, and scd1Δ rga4Δ cells treated with 12 mM HU for 5 h. (B) DIC images from wild-type (top) and scd1Δ mutant (bottom) strains expressing rga6+ (left) or rga4+ (right) from the nmt1 promoter. Cells grown in EMM without thiamine for 22 h at 28°C. (C) Rga6-GFP localization in wild-type and scd1Δ cells treated with 12 mM HU for 5 h. Bar, 5 μm.
