Figure 5. Result from the 3D reconstruction of 20S proteasome from an in-focus dataset acquired with the Volta phase plate.
(A) An isosurface representation of the density map. (B) A ribbon and a stick representations of an α-helical segment from the β subunit docked into the density map demonstrating the presence of sidechain densities. (C) Fourier shell correlation (FSC) curves from the Relion software’s internal 'gold standard' and from a comparison with an external 2.8 Å density map (EMD-6287). Both criteria indicate a resolution of 3.2 Å at a 0.143 level for the 'gold standard' FSC and at a 0.5 level for the external map. The Nyquist frequency is at 2.7 Å (0.37 1/nm).