WT control, and NOX2−/− platelets, (3 × 108/mL) were stimulated with 0.018 U/mL thrombin, 150 nM U46619 or 0.5 µg/mL CRP. (A) Platelet aggregation and ATP secretion traces. (B) Quantification of platelet aggregation. (C) Quantification of platelet secretion. ***, ** and * represents P<0.001, P<0.01 and P<0.05, respectively as determined by Student’s t-test (n=3).