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. Author manuscript; available in PMC: 2017 May 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2016 Mar 24;36(5):984–993. doi: 10.1161/ATVBAHA.115.306140

Figure 1. Drebrin gene trap reveals Drebrin expression in SMCs.

Figure 1

A, Design of the Dbn targeting construct used to generate Drebrin KO mice. SA, splice acceptor; IRES, internal ribosome entry site; βgal, β-galactosidase gene; polyA, polyadenylation sequence; FRT, Flp recombinase target. B, Brain lysates from WT and Dbn−/− neonatal mice were subjected to sequential immunoblotting for Drebrin and then tubulin, as indicated. C-D, Aortas from 8-wk-old mice of the indicated genotype were subjected to X-gal staining and (C) photographed at 5× magnification or (D) paraffin-embedded, sectioned, and counterstained with eosin. E, The following mouse cells were solubilized, and 30 μg of cell protein was subjected to serial immunoblotting for Drebrin and tubulin: primary SMCs, primary endothelial cells (EC), RMA lymphoma cells (RMA), and primary macrophages (MP). F, Thirty μg of protein from WT or Dbn−/+ SMCs were immunoblotted sequentially for Drebrin and then tubulin.