Table 2.
Results of immunohistochemical and genetic test
| Case | Positive immunohistochemical stains | Negative immunohistochemical stains | Genetic confirmation |
|---|---|---|---|
| 1 | CD99 | N/A | ND |
| 2 | CD99, NSE, vimentin | Desmin | ND |
| 3 | CD99, FLI1, vimentin, synaptophysin | NSE, S100, desmin, myogenin | ND |
| 4 | CD99, vimentin | CK7, CK20, AE1/3, LCA, S100, TTF-1, desmin, CD56, chromogranin | EWS-FLI1 fusion by PCR |
| 5 | CD99, FLI1 | CK7, CK20, AE1/3, LCA, synaptophysin, chromogranin, CD56, S100, actin, desmin, EMA, WT1 | EWSR1 rearrangement by FISH |
| 6 | CD99, FLI1 | CD3, TdT | EWSR1 rearrangement by FISH |
NSE – neuron specific enolase, CK – cytokeratin, LCA – leukocyte common antigen, TTF-1 – thyroid transcription factor – 1, TdT – terminal deoxynucleotidyl transferase, EMA – epithelial membrane antigen, WT1 – Wilms tumor 1, N/A – not applicable, ND – not done