Table1.

Summary of valuable references and guidelines relevant to clinical NGS oncology testing

Source		Title		Content summary		Reference
ACMG: American College of Medical Genetics and Genomics; CDC: United States Centers for Disease Control and Prevention
New York State Board of Health		"Next Generation" Sequencing (NGS) guidelines for somatic genetic variant detection		Detailed standards for technical validity		11
ACMG		Standards and guidelines for the interpretation of sequence Variants		Guidelines for clinical validity assessment, particularly for germline/constitutional variants		12
ACMG		ACMG clinical laboratory standards for next-generation sequencing		Broad summaries of major areas of consideration for clinical validation of all NGS assay		13
CDC		Assuring the quality of next-generation sequencing in clinical laboratory practice		Detailed recommendations for technical validity assessment/validation of all NGS assays		14
CDC		Good laboratory practice for clinical next-generation sequencing informatics pipelines		Detailed recommendations for clinical validity assessment for all NGS assays		15
Quest Diagnostics (reference laboratory)		Annotation of sequence variants in cancer samples processes and pitfalls for routine assays in the clinical laboratory		The framework for a repeatable workflow for clinical validity assessment in use at a high volume testing facility is described		16