Table 2.
Primary antibodies used for immunohistochemical stainings
| Antibody | Manufacturer | Product Code | Dilution |
|---|---|---|---|
| Wnt2 | R&D, Minneapolis, MN | AF3464 | 1:50 |
| Wnt4 | Sigma-Aldrich, Zwijndrecht, The Netherlands | HPA011397 | 1:50 |
| Wnt7b | Sigma-Aldrich, Zwijndrecht, The Netherlands | SAB2104506 | 1:200 |
| R-Spondin2 | Santa Cruz Biotechnology, Santa Cruz, CA | SC-74,883 | 1:100 |
| Sfrp4 | Sigma-Aldrich, Zwijndrecht, The Netherlands | HPA009712 | 1:25 |
| Sulf1 | Santa Cruz Biotechnology, Santa Cruz, CA | SC-98,325 | 1:100 |
| β-catenin | BD Transduction Laboratories, San Diego, CA | 610,153 | 1:100 |
| α-SMA | Dako, Glosstrup, Denmark | M0851 | 1:100 |
| Collagen-I | Abcam, Cambridge, MA | 6308 | 1:5000 |
| Collagen-III | Abcam, Cambridge, MA | 6310 | 1:5000 |