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. 2016 Apr 29;7:100. doi: 10.3389/fphar.2016.00100

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Copyright © 2016 Eid, Ito and Otsuki.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Enhanced mitophagy in hepatocytes of ETRs. (A,B) TEM of control (A) and ETRs (B). The short black arrows indicate mitophagosomes, while the long black arrows show mitophagolysosomes. (C,D) IEM of Parkin in control (C) and ETRs (D). Yellow arrows indicate 25 nm Parkin immunogold particles. (E,F) Immunofluorescence double labeling of Parkin (Red) and cytochrome c (green) in control (E) and ETRs (F). The white arrows show colocalization signals on merging (yellow) in magnified areas below. Note that DAPI (blue) is for nuclear counterstaining.