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. 2016 Apr 29;7:100. doi: 10.3389/fphar.2016.00100

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Copyright © 2016 Eid, Ito and Otsuki.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Parkin translocation to mitochondria and mitophagosomes with accumulated 8-OHdG in hepatocytes of ETRs. (A) Control while (B–D), ETRs. The boxed areas in C and D are magnified on the right. The long and short arrows indicate Parkin (25 nm) and 8-OHdG (6 nm) immunogold particles, respectively. M, mitochondrion. The method of post-embedding immunogold double labeling of Parkin (NB100-91921) and 8-OHdG (N45.1) was performed according to the manufacturer’s protocols (Aurion, Wageningen, Netherlands; http://www.aurion.nl/products/gold_sols.php) and recent publications (Eid et al., 2013a, 2016).