Fig. 4.

Fluorescence titration of M²¹K-TMR-hepcidin with Cu^II. a M²¹K-TMR-hepcidin (0.5 µmol/L) in MOPS (0.25 mol/L, pH 7.45): acetonitrile (50:50) was quenched by the addition of Cu^IISO₄ (2 µmol/L) in 50 µL aliquots; b Analysis of the same titration, monitoring the fluorescence at 578 nm