Fig. 2. IGFBP3, a geneup-regulated by hypoxia in the presence of serum.
RNA were extracted from Glio6 cells cultured in normoxia (Glio6: 3% O2) or hypoxia (0.3% O2) for 48 hours. Glio6 cells were cultured either in the presence or absence of serum. RNA were then processed for transcriptomic analyses (abbreviation used FCS: fetal calf serum).
A:Expression levels are expressed as arbitrary units corresponding to the hybridization signal values on Affymetrix Gene chips. * p≤0.05.
B: RT-qPCR analysis was performed with primers specific for IGFBP3. Data were normalized to β-actin * p≤0.05