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. Author manuscript; available in PMC: 2016 May 9.
Published in final edited form as: J Neurooncol. 2013 Mar 31:1124. doi: 10.1007/s11060-013-1124-4

Fig. 3. VE-cad, VE-PTP and CD133, three genes up-regulated by hypoxia in Glio6 cells, but whose expression is poorly detectable in U87 cells even under hypoxia.

Fig. 3

RNAs were extracted from cells cultured in normoxia (U87: 20% O2; Glio6: 3% O2) or hypoxia (0.3% O2) for 48 hours. Glio6 cells were cultured either in the presence or absence of serum. RNAs were then processed for transcriptomic analyses.

A: Expression levels are expressed as arbitrary units corresponding to the hybridization signal values obtained with Affymetrix arrays. * p ≤0.05; ** p≤ 0.01; # values below background.

B:RT-qPCR analysis was performed with primers specific for VE-cad, VE-PTP, CD133. Expression levels were normalized relative to the expression level of β-actin. Values are calculated by 2−(Ctgene of interest − Ctbeta actin) * p≤ 0.05; ** p≤0.01.