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. 2016 Apr 26;110(8):1811–1825. doi: 10.1016/j.bpj.2016.03.001

Figure 6.

Figure 6

Effects of MARCKS and PKCα on PI3Kα lipid kinase activity. Regulation of PI3Kα activity was quantified using the single-molecule TIRF assay at 21.5°C ± 0.5°C on standard PE/PS/DAG/PIP2 supported bilayers. (A) Time course of PIP3 production by PI3Kα, illustrating the effects of PKCα and MARCKS peptide (MARCKSp, the isolated PIP2-binding domain of MARCKS) on the net production of product PIP3 molecules per TIRF field. (B) Slopes of the time courses in (A), showing that PKCα largely restores the PI3Kα lipid kinase activity that is lost in the presence of MARCKSp, but PKCα has little or no effect on PI3Kα activity in the absence of MARCKSp. Single-molecule TIRF conditions as detailed in the Fig. 4 legend.