Fig. 3.

Assay sensitivity, efficiency, linearity and reproducibility.

The probes were tested in q-PCR assays using serial dilutions of DNA extracted from CVI988-infected CEF or RB-1B-infected CEF. Mean 40-Ct values from triplicate assays, with 95% confidence limits for the regression and the R² value, are shown for (a) pp38-CVI probe with serial dilutions of CVI988CEF DNA, and (b) pp38-Vir(3) probe with serial dilutions of RB-1B CEF DNA. The underlying points for 40-Ct values from triplicate assays are shown using three different symbols to distinguish the three assays (open squares, black diamonds and grey triangles).