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. 2016 Apr 19;7(2):e00345-16. doi: 10.1128/mBio.00345-16

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Copyright © 2016 Nölling et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 2 — Panel γPNA probe specificity validation. The specificities of the 17 panel γPNA probes, labeled I to XVII, were evaluated against 16S/18S amplicons (1 fmol) generated from all 21 target organisms. (a) Representative results for E. aerogenes, S. agalactiae, and C. glabrata. Note the high signals for targeted organisms against low background signals derived from nontarget organisms. (b) Distribution of off-target signals as a fraction of target signal generated by all 17 γPNAs and all 21 panel organisms. Quantification showed a typical off-target signal of about 0.43%, indicating a typical signal/off-target ratio in excess of 200×. All studies were completed in triplicate; data are presented as means ± SD.