FIG 1 .

Establishment of an O-linked glycosylation system in the attenuated S. flexneri strain 301DWP. (A) Silver staining of LPS from S. flexneri 2a strains 301 and 301DWP. (B and C) Coomassie blue staining and Western blot assays to analyze the glycosylation of the natural substrate protein, PilE (B), or of recombinant substrates, CTB protein fused with the PilE S⁴⁵-K⁷³ fragment (4573) at the C terminus or recombinant rEPA or TTc proteins fused with 4573 at the N terminus (C), when each was coexpressed with the corresponding O-linked glycosyltransferase PglL.