FIG 6 .

Mutation of the cysteine residues that coordinate the WhiB [4Fe-4S] cluster prevents DNA binding in vivo. Anti-FLAG ChIP-seq data for WhiB and WhiB (4C-S) are shown for three representative WhiA and WhiB target genes: ftsW, ftsZ, and filP. Color coding of the ChIP samples is as follows: 3×FLAG-[Gly₄Ser]₃-WhiB strain (WhiB-FLAG), red; 3×FLAG-[Gly₄Ser]₃-WhiB(4C-S) strain, green; corresponding S. venezuelae wild-type anti-FLAG negative control (WT), blue. Plots span approximately 3 kb of DNA sequence. Genes running right to left are shown in red. The black arrow indicates the gene subject to WhiA and WhiB regulation.