TABLE 1 .

Summary of thermodynamic parameters for wild-type SGBP-A and SGBP-B obtained by isothermal titration calorimetry at 25°C^a

Carbohydrate	Ka (M−1)		ΔG (kcal ⋅ mol−1)		ΔH (kcal ⋅ mol−1)		TΔS (kcal ⋅ mol−1)
	SGBP-A	SGBP-B	SGBP-A	SGBP-B	SGBP-A	SGBP-B	SGBP-A	SGBP-B
XyGb	(4.4 ± 0.1) × 105	(5.7 ± 0.2) × 104	−7.7	−6.5	−14 ± 3	−14 ± 2	−6.5	−7.6
XyGO2c	3.0 × 105	2.0 × 104	−7.5	−5.9	−17.2	−17.6	−9.7	−11.7
XyGO1	NBd	(2.4 ± 0.1) × 103	NB	−4.6	NB	−4.4 ± 0.2	NB	0.2
Cellohexaose	568.0 ± 291.0	NB	−3.8	NB	−16 ± 8	NB	−12.7	NB
Cellotetraose	NB	NB	NB	NB	NB	NB	NB	NB

^aShown are average values ± standard errors from two independent titrations, unless otherwise indicated.

^bBinding thermodynamics for XyG based on the concentration of the binding unit, XyGO₂.

^cValues from a single titration.

^dNB, no binding observed.