TABLE 1.
Values for reaction rate parameters used for simulations
| Reaction | Value | Reference |
|---|---|---|
| Formation of presumo | bps: 6.286e-3 μm/h | Ref. 13 |
| Degradation of presumo | dps: 2.218e-2 h−1 | Assumed to be same as ds |
| Degradation of mature SUMO | ds: 2.218e-2 h−1 | Ref. 13 |
| Formation of E1 | bE1: 2.426e-2 μm/h | Ref. 13 |
| Degradation of E1 | dE1: 4.313e-3 h−1 | Ref. 13 |
| Formation of E20 | bE20: 4.416 e-2 μm/h | Ref. 13 |
| Degradation of E20 | dE20: 4.132e-3 h−1 | Ref. 13 |
| Formation of SENP | bSENP: 3.212e-4 μm/h | Ref. 13 |
| Degradation of SENP | dSENP: 3.454e-2 h−1 | Ref. 13 |
| Formation of the target (RanGAP1) | bT10: 4.591e-2 μm/h | Ref. 13 |
| Degradation of target (RanGAP1) | dT10: 1.938e-2 h−1 | Ref. 13 |
| Degradation of sumoylatedtarget | dT11: 1.938e-2 h−1 | Assumed to be same as dT10 |
| Preprocessing of presumo | Km: 27.9 ± 3.7 μm | Ref. 22 |
| kcat: 0.72 ± 1.5 s−1 | ||
| Activation: transfer of SUMO on E1 | kcat: 0.78 ± 0.49 s−1 | Ref. 24 |
| Km: 0.17 ± 0.096 μm | ||
| Conjugation: transfer of SUMO to E20 | kcat/Km: 1.06 ± 0.05 μm−1 s−1 | Refs. 12 and 25 |
| Ligation of target (RanGAP1) by E20 | kcat: 0.66 ± 0.14 s−1 | Ref. 26 |
| kd: 2.9 ± 0.96 μm | ||
| Deconjugation of modified target (RanGAP1) | Km: 33.1 ± 3.2 μm | Ref. 22 |
| kcat: 50.4 ± 2.2 s−1 |