FIGURE 5.

PNT1 is exclusively required for kinetoplast function. A, left panel, cumulative growth curve of PNT1γL262P RNAi line over time ± tetracycline (n = 3). Right panel, the mRNA levels of PNT1 transcript were evaluated in the induced and uninduced PNT1γL262P RNAi cell line using quantitative RT-PCR. Each histogram represents the mean ± S.D. (n = 3). B, loss of kinetoplast in the PNT1γL262P RNAi cell line after addition of tetracycline to down-regulate PNT1. The cells were grown for 120 h ± tetracycline and stained with DAPI. Scale bar, 5 μm.