FIGURE 8.

CypB binds to LHs and FKBP65. Binding assay in a cell culture system. 293F cells were transiently co-transfected with CypB or P21-Myc and LHs-FLAG or FKBP65-HA plasmids and subjected to co-immunoprecipitation (IP). After 48 h, the cells were lysed, and CypB or p21 protein complexes were immunoprecipitated from the lysates with the anti-Myc antibody or normal mouse IgG as a negative control. The pulldown of CypB or P21 was confirmed by Western blotting analysis with anti-Myc antibody, and the pull-down of LHs or FKBP65 proteins was confirmed with an anti-FLAG or HA antibody. A, interaction between CypB or P21 and LH1, -2, and -3. B, interaction between CypB and LHs by increasing concentrations of CypB. C, interaction between CypB or P21 and FKBP65. D, interaction between CypB and FKBP65 by increasing concentrations of CypB.