TABLE 1.

Hydroxylation of lysine and proline, glycosylation of hydroxylysine (residues/mole of collagen), and percentages of glycosylation in total hydroxylysine of WT, Het, and CypB KO mouse tendon collagen

Values represent mean ± S.D. (n = 10) of triplicate analysis of the hydrolysates. A, Lys hydroxylation was calculated as Hyl/collagen based on the value of 300 residues of Hyp/collagen. Pro hydroxylation was calculated by Hyp/(Pro + Hyp). B, glycosylation of hydroxylysine was calculated as residues/collagen. free, nonglycosylated Hyl. C, glycosylation of hydroxylysine (%) represents the relative levels of GG-Hyl, G-Hyl, and free Hyl (GG-Hyl + G-Hyl + free-Hyl = 100%).

*Significantly different from both WT and Het (p < 0.01).

**Significantly different from both WT and Het (p < 0.001).