Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Mar 3;291(18):9596–9609. doi: 10.1074/jbc.M116.716621

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 6. — A and B, ability of kynoxazine to cross-link and form fluorescent adducts in αB-crystallin; SDS-PAGE of αB-crystallin incubated under different conditions. A, Coomassie; B, fluorescence. Lane 1, molecular weight markers; lane 2, αB-crystallin alone without incubation; lane 3, αB-crystallin alone incubated; lane 4, αB-crystallin + 3OHKynG; lane 5, αB-crystallin + Kyn; lane 6, αB-crystallin + 3OHKyn; lane 7, αB-crystallin + erythrulose; lane 8, αB-crystallin + kynoxazine. C, Coomassie; D, fluorescence. Lane 1, molecular weight markers; lane 2, αB-crystallin alone incubated; lane 3, αB-crystallin + AG; lane 4, αB-crystallin + 3OHKyn; lane 5, αB-crystallin + 3OHKyn + AG; lane 6, αB-crystallin + erythrulose; lane 7, αB-crystallin + erythrulose + AG; lane 8, αB-crystallin + kynoxazine; lane 9, αB-crystallin + kynoxazine + AG. E, Coomassie; F, fluorescence. Lane 1, molecular weight markers; lane 2, αB-crystallin alone; lane 3, acetylated αB-crystallin alone; lane 4, αB-crystallin + 3OHKyn; lane 5, acetylated αB-crystallin + 3OHKyn; lane 6, αB-crystallin + erythrulose; lane 7, acetylated αB-crystallin + erythrulose; lane 8, αB-crystallin + kynoxazine; lane 9, acetylated αB-crystallin + kynoxazine. The bar graphs on the right show the combined densitometric values of all high molecular mass protein bands (>25 kDa), and each bar represents the mean ± S.D. (error bars) of three independent experiments. **, p = 0.05; ***, p = 0.005; NS, not significant.