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MS sequencing of major oligosaccharides of LMWG fraction from WT-BclA-Bc. Shown are MALDI-MS analysis (A) and reverse phase HPLC separation (B) of oligosaccharides of WT-BclA-Bc LMWG, MALDI-MS (C) and MALDI-MS/MS (D) of oligosaccharide at m/z 552 from reverse phase HPLC fraction at retention time 11.0 min, and MALDI-MS (E) and MALDI-MS/MS (F) of oligosaccharide at m/z 712.2 from reverse phase HPLC fraction at retention time 33.3 min. deHex, deoxyhexose.
