NMR analysis of major tetrasaccharide of LMWG fraction from WT-BclA-Bc. Shown is the 1H-13C HSQC spectrum of tetrasaccharide from WT-BclA-Bc LMWG. A, zoom of anomeric region of 1H-13C HSQC; B, zoom of methyl group chemical shift region; C, zoom of 1H-13C HMBC showing quaternary carbon of unit III; D, zoom of 1H-13C HSQC spectrum where skeleton proton and O-methyl groups resonate; E, deduced structure of the trisaccharide.