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. 2016 Feb 26;291(18):9666–9677. doi: 10.1074/jbc.M116.718171

FIGURE 8.

FIGURE 8.

Prediction of the CTD glycosylation sites. Putative sites of O-glycosylation on the C-terminal domain of B. anthracis and B. cereus BclA were inferred from the crystal structure of B. anthracis BclA (34). The best resolution crystal structure of the C-terminal domain of B. anthracis BclA (Protein Data Bank code 1WCK) was used to illuminate 16 O-glycosylable (Ser and Thr) residues supposed to be on the surface of BclA CTD from the top (A) and side (B). For clarity, only β-carbons are represented as spheres on one monomer of the trimer: red indicates surface-exposed Ser/Thr residues common to B. anthracis and B. cereus (92/96/109/119/142/146/152/169/183/184/185/194/199 in B. anthracis numbering); yellow and magenta indicate those present in only B. anthracis (144/166) and B. cereus (159), respectively. The end of the collagen-like domain anchored in the basal layer is at the bottom of the image. The image was made by MOLMOL software (53). C, hypothetical representation of the domain-specific glycosylation of BclA.