TABLE 2.
Proton and carbon chemical shifts of major trisaccharide 3-O-Me-Rha(α1–2)Rha(α1–3)GalNAc-ol isolated from LMWG WT-BclA-Bc (Fig. 3)
Bold values indicate substitution positions.
| 1 | 2 | 3 | 4 | 5 | 6,6′ | Add -CH3 | ||
|---|---|---|---|---|---|---|---|---|
| GalNAc-ol (ol) | 1H | ∼3.71 | 4.35 | 3.99 | 3.62 | 3.86 | ∼3.65 | 2.05a |
| 13C | 62.2 | 53.1 | 79.4 | 70.8 | 71.4 | 64.2 | 23.3 | |
| α-Rha (I) | 1H | 5.03 | 4.04 | 3.91 | 3.51 | 3.78 | 1.29 | No |
| 13C | 102.0 | 79.7 | 71.2 | 73.2 | 71.2 | 18.0 | No | |
| α-3-O-Me-Rha (II) | 1H | 5.05 | 4.35 | 3.49 | 3.48 | 3.76 | 1.29 | 3.44b |
| 13C | 103.6 | 67.1 | 80.6 | 72.3 | 70.5 | 18.0 | 57.2 |
a Methyl group from N-acetyl group.
b Methyl group carried by an oxygen.