TABLE 3.
Proton and carbon chemical shifts of major tetrasaccharide Cro(β1–4)3-O-Me-Rha(α1–2)Rha(α1–3)GalNAc-ol isolated from LMWG WT-BclA-Bc (Fig. 4)
Bold values indicate substitution positions.
| 1 | 2 | 3 | 4 | 5 | 6,6′ | Add -CH3 | ||
|---|---|---|---|---|---|---|---|---|
| GalNAc-ol (ol) | 1H | ∼3.7 | 4.34 | 3.98 | 3.62 | 3.86 | ∼3.65 | 2.05a |
| 13C | 62.3 | 53.1 | 79.5 | 70.9 | 71.3 | 64.2 | 23.4 | |
| α-Rha (I) | 1H | 5.03 | 4.04 | 3.90 | 3.51 | 3.78 | 1.30 | No |
| 13C | 102.0 | 79.7 | 71.2 | 73.2 | 71.1 | 18.0 | No | |
| α-3-O-Me-Rha (II) | 1H | 5.05 | 4.35 | ∼3.7 | ∼3.7 | 3.83 | 1.37 | 3.45b |
| 13C | 103.3 | 67.0 | ∼80.7 | ∼80.7 | 69.2 | 18.4 | 57.2 | |
| β-3-C-Me-6-dGul (III) | 1H | 4.79 | 3.37 | No | 3.27 | 4.18 | 1.22 | 1.36c |
| 13C | 103.9 | 74.0 | 74.9d | 76.7 | 71.1 | 16.8 | 23.5 |
a Methyl group from N-acetyl group.
b Methyl group carried by an oxygen.
c Methyl group carried by a carbon.
d Value obtained on HMBC experiment through 3JH,C.