Skip to main content
. 2016 Mar 7;291(18):9690–9699. doi: 10.1074/jbc.M115.707612

FIGURE 1.

FIGURE 1.

Regulation of TERT mRNA expression levels by ARID1A. A and B, quantitative real time PCR demonstrates (A) silencing of ARID1A and (B) up-regulation of TERT mRNA expression after knockdown by siARID1A in hEM3 endometrial epithelial cells and HEK293FT embryonic kidney cells. CRISPR-Cas9 genome editing of ARID1A increases TERT mRNA expressions expression in hEM3 ARID1A KO cells. Reintroduction of ARID1A in ARID1A-null ovarian clear cells (OVISE and OV207) leads to (A) up-regulation of ARID1A and (B) down-regulation of TERT mRNA expression. β-actin (ACTB) was used as an internal loading control. C, immunoblot analysis demonstrating changes in TERT expression following modification of ARID1A expression using siRNA, CRISPR/Cas9, and Tet-on inducible systems. GAPDH was used as a protein loading control. The data are expressed as means ± S.E.; three independent experiments were performed. **, p < 0.01; ***, p < 0.001.