FIGURE 3.

ARID1A affects TERT promoter activity by direct binding to the TERT promoter region. A, the human TERT promoter luciferase reporter plasmid was co-transfected with pRL-Renilla construct into nontransformed human epithelial cells (hEM3 and 293FT) with ARID1A knockdown, and ARID1A mutated ovarian clear cells (OVISE and OV207) with ARID1A induction. B and C, ChIP-qPCR was performed using anti-ARID1A antibody (HPA005456, Sigma-Aldrich) (B) and anti-V5 antibody (ab15828, Abcam) (C) in both ARID1A-induced (+ Tet) and noninduced (DMSO) cells. CDKN1A (p21) and CHGA promoters were included as a positive and a negative control, respectively. The data were obtained from triplicate experiments and are expressed as means ± S.E. *, p < 0.05; ***, p < 0.001.