FIGURE 4.

ARID1A-SIN3A co-repressor complex is localized at the TSS region of TERT promoter. A–D, ARID1A expression was induced by incubating the OVISE ARID1A-inducible cells with 1 μg/ml doxycycline for 48 h (+Tet), and DMSO was used as a control on a separate group. ChIP assays were performed to examine the occupancy of SIN3A (NB600-1263, Novus Biologicals) (A), histone 3 lysine 9 trimethylation (H3K9me3, ab8898, Abcam) (B), histone 4 lysine 12 acetylation (H4K12Ac, 07-595, Millipore) (C), and RNA Pol II (ab5095, Abcam) (D). Immunoprecipitated DNA samples were quantified by qPCR, and their enrichment were expressed as percentage of the input. E, Pearson's correlation analysis demonstrated a significant negative relationship between ARID1A and TERT mRNA expression in ovarian clear cell carcinoma tissues.