FIGURE 9.

The distributions of 5′NT, but not MRP2, are altered in cells overexpressing K68R rab17. Uninfected cells (con) and WIF-B cells expressing FLAG-tagged wild type (WT), K68R, or K41R rab17 were fixed and stained for rab17 (not shown) or endogenous MRP2 (A) or 5′NT (C). Arrows mark the intracellular, subapical 5′NT labeling in cells overexpressing K68R rab17. The images were intentionally overexposed to highlight the subapical labeling. Bar = 10 μm. In B and D, the percent of infected cells were scored for intracellular subapical staining and the percent of total infected cells with such staining was calculated. Values are expressed as the average from at least 3 independent experiments ± S.E. *, p ≤ 0.001.